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KMID : 0357319930280010069
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Journal of the Korean Society for Microbiology
1993 Volume.28 No. 1 p.69 ~ p.80
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Effect of 1,25 Dihydroxy-vitamin D3 and Physiological Inducers on the Expression of Interleukin-1beta Gene in the Human Myeloid Leukemic Cells
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오종석
강인철/정 진/신부안/송명자/이현철
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Abstract
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The effect of 12-0-tetradecanoyl phorbol-13-acetate (TPA), 1,25 dinhydroxy-vitamin D3 (DHVD3), interferon-r (rIFN), dexamethasone alone or in combination on the degree of cellular differentiation and the level of interleukin-1β(IL-1β)
expression
was
assessed in human myelomonocytic leukemic cell line, U-937, by morphological observation, flow cytometry and Northern blot hybridization.
@EN 1. The TPA-treated U-937 cells showed to be gathered, to showed to be gathered , to shape irregularly and to die, while either DHVD3-, rIFN, or dexamethasone-treated cells showed no change in the morphology. Among three combinations of TPA
and
other
agents, the cells treated with combination of TPA and DHVD3 were partly changed into logn cells. Compared with control, the proliferation and cell viability of U-937 cells treated with TPA, or combination of TPA and DHVD3 or rIFN was suppressed.
2. The mean fluorescence intensity of CD14 molecules on the cell surface and the percentage of the CD14+ cells were increased up to 78.66 and 61.2%, respectively, in the cells treated with combination of Tpa and DHVD3 in comparison with the
control
cells (6.17, 2.9%). The mean fluorescence intensity of CD15 molecules on the cell surface and the percentage of the CD15+ cells were decreased to 42.73 and 2.5%, respectively, in the cells treated with combination of TPA and rIFN in comparison
with
the
control cells (292.09, 51.3%).
3. Poly (A+) MRNA was extracted from cells treated with the various agents along or in combination for 18 hours. Northern blot analysis showed that compared with TPA-treated cells, the IL-β expression of cells treated with combination of TPA and
DHVD3
significantly increased, while that of cells treated with combination of TPA and rIFN or dexamethasone decreased, while that of cells treated with combination of TPA and rIFN or dexamethasone decreased.
4. Culture media of cells treated with the various agents alone or in combination for 48 hours was assessed for IL-1 activity. Compared with control cells, cells treated with TPA alone or combination of TPA and dhvd3 or rIFN showed some IL01
activity.
These results indicate that the differentiation of U-937 cells may be related considerably to the increase of CD14 antigen expression and IL-1β gene expression.
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KEYWORD
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